Place solvent cabinet with the solvent bottles always on top (or at a higher level) of the pump.
Flush the degasser with at least 5 mL per channel before operating the pump, especially when the pumping system had been turned off for a certain length of time (for example, overnight) and volatile solvent mixtures are used in the channels.
Prevent blocking of solvent inlet filters (never use the pump without solvent inlet filters). Growth of algae should be avoided (see Prevent Blocking of Solvent Filters).
Check purge valve frit and column frit in regular time intervals. A blocked purge valve frit can be identified by black, yellow or greenish layers on its surface or by a pressure greater than 10 bar in low delay volume configuration and 20 bar in standard configuration when pumping distilled water at a rate of 5 mL/min with an open purge valve.
Whenever possible use a minimum flow rate of 5 µL/min per solvent channel to avoid crossflow of solvent into the unused pump channel.
Whenever exchanging the pump seals, the purge valve frit should be exchanged, too.
When using buffer solutions, flush the system with water before switching it off. The seal wash option should be used when installed, especially when buffer solutions with concentrations of 0.1 M or higher are being pumped for long periods of time.
Check the pump pistons for scratches, grooves and dents when changing the piston seals. Damaged pistons cause micro leaks and will decrease the lifetime of the seals.
After changing the piston seals, apply the seal wear-in procedure (see Seal Wear-in Procedure).
Place the aqueous solvent on channel A and the organic solvent on channel B. The default compressibility settings are set accordingly.
base-id: 3847232267
id: 3847232267