Always use freshly prepared solvents, especially use demineralized water, which was filtered through 0.2 µm filters.
Never leave mobile phase in the instrument for several days without flow.
Always discard old mobile phase.
Use the amber solvent bottle (9301-6526 (Solvent bottle, amber, 1000 mL) ) supplied with the instrument for your aqueous mobile phase.
If possible add a few mg/L sodium azide or a few percent organic solvent to the aqueous mobile phase.
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