The Multisampler processor continuously monitors the movements of the Multisampler components. The processor defines specific time windows and mechanical ranges for each movement. If a specific step of the sampling sequence is not completed successfully, an error message is generated. Solvent is bypassed from the Multisampler by the injection valve during the sampling sequence. After the required sample container is automatically loaded from the sample hotel, it is placed on the central workspace. The needle assembly moves via a robot to the desired sample position. Then it is lowered into the sample liquid to allow the metering device to draw up the desired volume by moving its plunger back a certain distance. The needle assembly is then raised again and moved to the needle park station onto the seat to close the sample loop. Sample is applied to the column when the injection valve returns to the mainpass (main path) position at the end of the sampling sequence.
The standard sampling sequence occurs in the following order:
The robot loads the required sample container on the central workspace.
The injection valve switches to the bypass position.
The plunger of the metering device moves to the initialization position, pushing out clean eluent through the waste tube (port 4) for a standard or through the needle for a Multiwash system.
The robot couples into the needle assembly from the needle parkstation.
The robot unlocks the needle assembly and moves up.
The coupled needle assembly/robot moves to the desired sample vial (or well plate) position on the central workstation.
The needle lowers into the sample vial (or well plate).
The metering device draws the preset sample volume.
The needle lifts out of the sample vial (or well plate).
The coupled needle assembly/robot is then moved to the park station onto the seat to close the sample loop.
The needle assembly is locked into the park station and moves down.
The injection cycle is completed when the injection valve switches to the mainpass (main path) position.
The robot moves the sample container back into the sample hotel after the sampling sequence is done. If needle wash is required, it will be done between step 9 and 10.
NOTE
For the needle seat backflush, the Multisampler must be in bypass mode.
If an additional needle seat backflush is required, this step must also be done between step 5 and 9.
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