Make sure all supplies (fittings, capillaries, inline filters, columns, etc.) are bio-inert / biocompatible.
Be aware that some columns for bio-related applications have a stainless steel case and can introduce iron and other metal ions in the flow path. This may lead to adsorption of susceptible samples like phosphorylated nucleotides. In this case, use PEEK-lined columns.
After using the system with high salt concentrations, flush it extensively with water to prevent blockages caused by salt crystals.
Reliable operation of 1290 pumps during analysis cannot be guaranteed if pressure falls below 20 bar. For optimal results, pressure should be at least 50 bar continuously. Therefore, when using columns that create low backpressure (< 50 bar, such as SEC columns with 1290 LC systems), install a Restriction 5005-0046 (Capillary MP35N 0.12 mm x 2 m) between the pump and the sampler, to achieve at least 50 bar.
Perform daily flush of the Multisampler with water if the Multiwash option is installed (see Best Practices for Multiwash).
CAUTION
Agilent Bio-inert and Bio LC systems should not be subject to passivation or similar procedures
This can cause irreversible damage to the system's internal surfaces
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