The Agilent 1290 Infinity I/II/III Pumps are equipped with automatic purge valves. This enables many extra functions not available in Agilent Pumps with manual purge valves. It is possible to prepare the pump (set parameters and start the functions Purge, Condition, or Prime) with the software.
Use the Purge function to:
Fill the system with fresh or different solvent.
Ensure that the new solvent is miscible with the previous solvent.
Prevent damage to the degasser or pump by using an intermediate step with a comiscible solvent, if necessary.
Remove air bubbles in tubes and pump heads.
After the pump has been idle for a few hours or more (air may have diffused into the solvent lines).
When the purge procedure ends, the module automatically switches to analytical conditions again.
If micro air bubbles persist in the pump head, the overall pump performance may be compromised and flow accuracy / precision may be negatively affected. This may be visible as increased pressure and / or detector baseline ripple. A reliable indicator of such situation is a strongly negative but slowly increasing tuning signal value (below –1). To remove the air efficiently, the Condition function can be used. During conditioning, the pump is delivering flow into the system (column) and the last used method settings, like flow, composition and max pressure are used. It is not possible to perform sample analysis while conditioning.
Use a reasonable flow rate (for example 1.5 mL/min), composition setting (for example A: 50 % B: 50 %) and backpressure (> 200 bar) to ensure efficient air bubble removal from all pump heads.
Condition the pump if you see:
Excessive pressure ripple.
Excessive composition ripple (baseline noise/mixing noise – noise level changes with the composition), when you are sure that the solvent type is correctly set, and there is no evidence of a leak in the pump.
Conditioning may be necessary:
After a long period of standby
After running out of solvent
After service or repair
CAUTION
Filling empty solvent lines
Damage to the seals
The Prime function is helpful if air has entered the pump heads and cannot be removed by conditioning for 15 minutes. The module draws solvent at a high speed with all pump drives simultaneously and dispenses it into the waste position of the automatic purge valve. This is done 20 times and is stressful to the valve and rotor seal. Therefore, it should be performed only as a last resource, before forcefully filling the pump heads with a syringe or attempting to repair the pump heads.
Use the Prime function to:
Free a potentially stuck valve.
The described functions can be triggered from the driver interface:
1290 Infinity
where
1 | Right click the module dashboard |
2 | Select the appropriate function to start the procedure |
where
1 | Right click the module dashboard |
2 | Select Prepare Pump... |
3 | Select the procedure and fill in adequate parameters |
4 | Click Start to run the selected procedure |
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