Flush the Flow Cell
When
|
Tools required
Qty. | p/n | Description | |
|---|---|---|---|
1 | 
|
| Glass syringe |
1 |
|
| Adapter |
Parts required
Qty. | p/n | Description | |
|---|---|---|---|
1 |
|
| Strong solvent |
1 |
|
| Tubings to waste |
WARNING
Dangerous solvents
The strong solvents used in this procedure are toxic and flammable and proper precautions are necessary.
- Wear protective gloves and goggles.
- Don't expose yourself to the vapors.
NOTE
Aqueous solvents can build up algae and therefore should do not be left in the flow cell for long periods. Add a small percentage of organic solvents (e.g. acetonitrile or methanol ~ 5 %).
NOTE
The strong solvent should dissolve any potential contaminants in the flow cell. For example water for aqueous mobile phase buffers, chloroform or tetrahydrofuran for not water soluble contaminants.
NOTE
Do not exceed the flow cell pressure limit of 5 bar (0.5 MPa).
In case the cell is contaminated, follow the procedure below.
-
Use the purge mode and flush with the strong solvent.
-
Leave this solution in the cell for about one hour.
-
Flush with mobile phase.
base-id: 3596214795
id: 3596214795