Flow Cell Flushing
When
|
Tools required
Qty. | p/n | Description | |
|---|---|---|---|
1 | 
|
| Glass syringe |
1 |
|
| Adapter |
Parts required
Qty. | p/n | Description | |
|---|---|---|---|
1 |
|
| Bidistilled water, nitric acid (65 %), tubings to waste |
WARNING
Dangerous concentration of nitric acid
The nitric acid flushing procedure is not an infallible remedy for a dirty cell. It is to be used as a last attempt to salvage the cell before cell replacement. Note that the cell is a consumable item.
- Give proper attention to safety.
NOTE
Aqueous solvents in the flow cell can built up algae. Algae do fluoresce. Therefore do not leave aqueous solvents in the flow cell for longer periods. Add a small percentage of organic solvents (e.g. Acetonitrile or Methanol ~5 %).
-
Flush with bidistilled water.
-
Flush with nitric acid (65 %) using a glass syringe.
-
Leave this solution in the cell for about one hour.
-
Flush thoroughly with bidistilled water at a higher flow rate, e.g. 1.5 mL/min.
NOTE
Do not exceed the pressure limit of 20 bar (0.2 MPa).
NOTE
Do not use the HPLC pump to pump nitric acid through the system.
base-id: 3587002635
id: 3587002635