Before you start you should check that your detector is performing according to the specifications published by Agilent Technologies.
Your normal LC grade solvents may give good results most of the time but our experience shows that baseline noise can be higher with LC grade solvents than with fluorescence grade solvents.
Flush your solvent delivery system for at least 15 minutes before checking sensitivity. If your pump has multiple channels, you should also flush the channels not in use.
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