Extra-column volume is a source of peak dispersion that will reduce the resolution of the separation and so should be minimized. Smaller diameter columns require proportionally smaller extra-column volumes to keep peak dispersion at a minimum.
In a liquid chromatograph the extra-column volume will depend on the connection tubing between the autosampler, column and detector; and on the volume of the flow cell in the detector. The extra-column volume is minimized with the Agilent InfinityLab LC Series system due to the narrow-bore (0.12 mm i.d.) tubing, the low-volume heat exchangers in the column compartment and the flow cell in the detector.
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