1 Selection of flow cell | |
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2 Connection of flow cell | |
For flow rates from 0.5 mL/min connect column using the zero-dead-volume fittings of the detector. For small column i.d. (e.g 1 mm) the inlet capillary of the micro flow cell can be connected directly to the column.
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3 Setting the peak width (response time) | |
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4 Setting wavelength and bandwidth | |
Sample wavelength: Never miss a peak by the use of a browser wavelength like 250 nm with 100 nm bandwidth. Select specific wavelength with reduced bandwidth if you need selectivity, e.g. 250,10 nm and 360,100 nm as reference wavelength. Set the sample wavelength to a peak or valley in the spectrum to get best linearity for high concentrations.
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5 Setting the slit width | |
Use 4 nm slit for normal applications. Use narrow slit (e.g 1 nm) if your analytes have narrow absorbance bands and for high concentrations. Use a wide slit (e.g. 16 nm) to detect very low concentrations. Optimizing spectral acquisition (DAD only) Select spectra acquisition mode according to your needs. Set the spectral wavelength range (for colorless samples 190...400 nm is sufficient). Set step to 4 nm for normal use; set small step (and slit width) if high resolution of spectra with fine structure is wanted.
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