While the Agilent 1290 Infinity, 1290 Infinity II/III, and 1260 Infinity II/III Flexible Pumps guarantee a high performance with a wide variety of solvents, other solvents may cause harm to the pump or to the (U)HPLC system. No modifications are necessary when using standard reversed phase applications with water and other polar protic solvents in combination with most polar aprotic solvents. Normal phase applications work well with the modifications detailed in the section Normal Phase Applications.
If free halides, hydroperoxides, free radicals or strong, oxidizing acids are present, corrosion can occur in stainless steel systems. There are several solvents with which, under nonideal conditions, reactions can occur and these harmful compounds are generated. Prevent formation of reactive substances when using the following solvents:
Solutions of alkali halides and their respective acids (for example, lithium iodide, potassium chloride)
High concentrations of inorganic acids like sulfuric acid and nitric acid, especially at higher temperatures
Halogenated organic solvents or mixtures, which form radicals and/or acids (for example, chloroform, methylene chloride)
Chromatographic grade ethers, which can contain peroxides (for example, THF, dioxane, di-isopropylether)
Solvents containing strong complexing agents (for example, EDTA).
All these solvents can be used in Agilent (U)HPLC systems. See Best Practices for Addressing Problems Associated With Unstable Solvents in an (U)HPLC Environment (BestPractices_UnstableSolvents_TN_EN.pdf, SD-29000193), for recommendations on how to avoid damage to the instrument or separation column when working with these solvents.
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