The injection can be optimized for speed remembering that drawing the sample too fast can reduce the reproducibility. Marginal gains are to be made here as the sample volumes used tend towards the smaller end of the range in any case. A significant portion of the injection time is the time taken with the needle movements to and from the vial and into the flush port. These manipulations can be performed while the previous separation is running. This is known as overlapped injection and it can be easily turned on from the sampler setup screen in the control software. The sampler can switch the flow to bypass after the injection has been made and then after, for example, 3 minutes into a 4 minutes run, start aspirating the next sample and preparing for injection. This can typically save 0.5 to 1 minute per injection.
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