Exchange a Flow Cell
|
|
For bio-inert modules use bio-inert parts only! |
When
|
Tools required
Qty. | p/n | Description | |
|---|---|---|---|
1 | 
|
| Wrench, 1/4 inch for capillary connections, OR |
1 | | 5043-0915 | Fitting mounting tool |
Parts required
Qty. | p/n | Description | |
|---|---|---|---|
1 | | G1321-60005 | Flow cell, 8 µL, 20 bar |
1 | | G1321-60015 | Flow cell, 4 µL, 20 bar, requires a 0.12 mm i.d. capillary (e.g. p/n G1316-87318, 300 mm long), part of Capillary kit for 0.12 mm id (p/n G1316-68716) |
1 | | G5615-60005 | Bio-inert flow cell, 8 µL, 20 bar includes Capillary Kit Flow Cells BIO (G5615-68755) and PEEK fittings |
1 | | G1321-60007 | FLD Cuvette Kit, 8 µL, 20 bar |
Prerequisites
|
CAUTION
Sample degradation and contamination of the instrument
Metal parts in the flow path can interact with the bio-molecules in the sample leading to sample degradation and contamination.
- For bio applications, always use dedicated bio parts, which can be identified by the bio-inert symbol or other markers described in this manual.
- Do not mix bio, and non-bio modules or parts in a bio system.
NOTE
New instruments are shipped with a flow cell installed and have a factory wavelength calibration.
If a flow cell is used other than the installed/shipped flow cell:
- verify that the installed flow cell is fixed very tight,
- perform a wavelength calibration using Glycogen (part of G7121-68001 (FLD Calibration Kit) ).
NOTE
Before storing the flow cell, flush it with iso-propanol and close it with 0100-1259 (Plug-Screw 1032- Fitting) .
-
-
-
-
-
Insert the flow cell and tighten the thumb screws strongly. NOTE: If the cell is not completely in its end position and tightened strongly this could result into a wavelength shift.
-
-
Reconnect capillaries.
-
Establish a flow and check for leaks.
-
-
Perform a Wavelength Calibration.
base-id: 3587044235
id: 18014402096526219