Installing MP35N Capillaries

This instruction describes swaging and installing MP35N capillaries.

The Agilent Bio LC systems use MP35N capillaries. These capillaries combine the high-pressure stability and bio-compatibility of MP35N. They are used throughout the complete system, including the flow path before and after sample introduction.

**Table:** Capillary connections for 1290 Infinity III Bio LC
p/n	From	To
G7120-60007 (Bottle Head Assembly, long (1730 mm))	Solvent Bottle	Infinity III Pump
5500-1419 (Capillary MP35N 0.17 mm x 500 mm, SI/SI)	Pump	Multisampler
5500-1279 (Capillary MP35N 0.12 mm x 500 mm SI/SI)	Multisampler	MCT
5500-1578 (Quick Connect Capillary MP35N 0.12 mm x 105 mm)	MCT Heat Exchanger	Column
5500-1596 (Quick Turn Capillary MP35N 0.12 mm x 280 mm)	Column/MCT Valve	Detector (DAD)
5500-1598 (Quick Turn Capillary MP35N 0.12 mm x 500 mm)	Column/MCT Valve	Detector (VWD)
5062-8535 (Waste accessory kit (Flow Cell to waste))	VWD	Waste
5062-2462 (Tube PTFE 0.7 mm x 5 m, 1.6 mm od)	DAD/FLD	Waste
G5664-68712 (Analytical tubing kit 0.25 mm i.d. PTFE-ESD)	Detector	Fraction Collector

Swage MP35N Capillaries

Use the wrench to turn the nut 90°.
Loosen the nut and verify that the ferrule is correctly positioned on the capillary: the front ferrule sits tight but still can be turned around the central axes, and the back ferrule should be sliding freely. If the front ferrule does not sit tight, repeat the procedure and at step 4 do about 5°-greater turn with the wrench than in the previous attempt.

Install MP35N Capillaries

Insert the fitting to the receiving port and push the capillary to the bottom of the port.
Finger tighten the nut into the port until snug.
With the wrench, tighten the nut until the first resistance. Make sure to pull the wrench instead of pushing it. When you can no longer pull the wrench with the fingers the first resistance position is reached.
Tighten the nut ¼ of a turn more with the wrench (⅛ of a turn for a second and subsequent installations).

21-Jun-2025