Online Spectra

In order to acquire spectra, a mode must be selected, either Excitation or Emission. Subsequently you define the scan range for the chosen mode and a wavelength for corresponding Emission or Excitation. Once all parameters for the Online Scan have been set, the Spectrum panel becomes active.

Peakwidth is a user-defined value representing the expected smallest chromatographic peak (in minutes). It helps the system set the appropriate data rate and is used to calculate Spectra per Peak, ensuring enough spectral data points are captured for accurate analysis.

The accepted range of values is 0.017 - 0.4 min (default: 0.025 minutes).

Step Size defines the interval between each wavelength point during a spectral scan. Smaller step sizes provide higher resolution but increase acquisition time.

Values 1 to 20 nm in steps of 1 nm are allowed (default: 10 nm).

Spectra per Peak indicates how many individual spectra are collected across a chromatographic peak during a scan. It is calculated based on the selected peak width. A higher number ensures better spectral resolution and more reliable identification of analytes.

NOTE: If the calculated value is less than 10, a warning will appear: "The selected peakwidth and scan range may not provide sufficient spectral resolution for reliable spectral analysis."