Spiky Peak Tops but Flat Baseline

1

Inconsistent nebulization

Nitrogen is the recommended gas - others can be used but may not nebulize as efficiently.

2

Incorrect gas being used

Change gas to nitrogen or evaluate different nitrogen sources.

3

Poor regulation of inlet gases

If using bottled gas, check that gas regulator is functioning correctly and giving consistent flow.

Alternatively, use a pulse dampener.

4

Insufficient smoothing

The broader the peaks, the higher the smoothing value is required. Increase smoothing to 50  for Flash separations.

5

Sample precipitation during nebulization

Reduce sample concentration or inject same loading in larger volume.

Check sample solubility in mobile phase eluents.

6

Inconsistent pump flow rates

See remedy for pump pulsation in the baseline noise section.