Wavelength Calibration
The wavelength calibration function verifies and corrects spectral accuracy using water Raman scattering. The system excites water in the flow cell with Hg-Xe lamp emission lines, generating Raman peaks at predictable wavelengths for calibration reference.
How It Works
The calibration uses four wavelength pairs across the detector range for enhanced precision:
312.9/350.1 nm
365.0/416.7 nm
404.7/469.2 nm
435.8/511.6 nm
The system uses Raman excitation maxima as reference values for the excitation monochromator (defined by the Hg-Xe emission line positions), while the corresponding Raman emission maxima calibrate the emission monochromator (shifted by ~3400 cm⁻¹Room temperature water recommended for optimal accuracy (±0.5 nm temperature sensitivity over the range of 5-45°C)). The system measures the Raman signal around each peak and uses 2D Gaussian fitting to determine precise peak positions. These measured positions are compared to reference values for verification or used to calculate new calibration factors.
Key Benefits
Simple operation: Uses standard HPLC-grade water.
Reliable reference: Based on fundamental physical constants—atomic emission lines and Raman shift.
Representative measurement: Signal comes from the same flow cell volume as the sample, eliminating calibration errors from localized light scattering.
Complete coverage: Fast calibration of both excitation and emission wavelengths simultaneously.
When
The detector gets calibrated at installation. Under normal operating conditions it should not require recalibration. However, it is advisable to recalibrate: |
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Parts required
Qty. | p/n | Description | |
|---|---|---|---|
1 |  | G7123-60500 | Flow Cell 2 µL |
1 | | G7123-60300 | Flow Cell 13 µL |
1 | | 0100-1259 | Plug-Screw 1032- Fitting |
Prerequisites
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Run the Wavelength Calibration with the Agilent Lab Advisor (for further information see Online-Help of user interface).
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